How many replicates per sample do I need to… A How many replicates per sample do I need to run tappAS? Users will need an RNA-Seq dataset with at least 2 replicates in order for tappAS to work. However, the more replicates, the better. Category: Application Should I use the FL counts file output by long-read processing tools, such as Cupcake and TAMA, as my count matrix? Videotutorial ISMB 2020