Can a use a transcriptome generated with Nanopore data… A Can a use a transcriptome generated with Nanopore data on tappAS? Yes, tappAS (as well as SQANTI3 and IsoAnnotLite) are fully compatible with Nanopore data as well as PacBio data. Category: Application I processed my long read samples separately, but tappAS requires one transcriptome for all samples. How do I generate one? How can I generate a transcript counts matrix?